Non-aqueous spray solvents and solubility effects in desorption electrospray ionization
نویسندگان
چکیده
منابع مشابه
Desorption Electrospray Ionization (DESI)
A new method of desorption ionization is described and applied to the ionization of various compounds, including peptides and proteins present on metal, polymer, and mineral surfaces. Desorption electrospray ionization (DESI) is carried out by directing electrosprayed charged droplets and ions of solvent onto the surface to be analyzed. The resulting mass spectra are similar to normal ESI mass ...
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The sampling rate and imaging capabilities of desorption electrospray ionization (DESI) are examined using a rotating sample platform combined with Hadamard transform time-of-flight mass spectrometry (HTTOFMS), a multiplexed time-of-flight technique that allows for millisecond acquisition of full mass-to-charge ratio scans. DESI-compatible dyes are used to produce spatially defined sample patte...
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Desorption electrospray ionization-mass spectrometry (DESI-MS) was evaluated for the detection of proteins ranging in molecular mass from 12 to 66 kDa. Proteins were uniformly deposited on a solid surface without pretreatment and analyzed with a DESI source coupled to a quadrupole ion trap mass spectrometer. DESI-MS parameters optimized for protein detection included solvent flow rate, temperat...
متن کاملEffects of matrix, electrospray solution, and laser light on the desorption and ionization mechanisms in electrospray-assisted laser desorption ionization mass spectrometry.
Electrospray-assisted laser desorption ionization (ELDI) is a technique which combines laser desorption with subsequent electrospray ionization. It is useful for directly detecting small and large molecules in solid or liquid samples under ambient conditions. In this paper, the detection of the protein molecules desorbed on a dry protein spot by using pulse laser energies of up to 300 microJ wa...
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Electrochemistry (EC) combined with mass spectrometry (MS) is a powerful tool for elucidation of electrochemical reaction mechanisms. However, direct online analysis of electrochemical reaction in aqueous phase was rarely explored. This paper presents the online investigation of several electrochemical reactions with biological relevance in the aqueous phase, such as nitrosothiol reduction, car...
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ژورنال
عنوان ژورنال: Journal of the American Society for Mass Spectrometry
سال: 2010
ISSN: 1044-0305,1879-1123
DOI: 10.1016/j.jasms.2009.12.012